Journal: bioRxiv
Article Title: Polysaccharide BAP1 of Bifidobacterium adolescentis CCDM 368 attenuates ovalbumin-induced allergy through inhibition of Th2 immunity in mice
doi: 10.1101/2024.09.14.613063
Figure Lengend Snippet: Systemic response after i.n. administration of BAP1 in the mouse model of allergic airway inflammation. A. Experimental design for BAP1 treatment, OVA sensitization and challenge. B. OVA-specific and total IgA, IgE, and IgG serum antibodies tested by ELISA. C. Cytokines and chemokines production in PBS- and BAP1-treated mice in response to restimulation with an allergen (OVA) measured in splenocyte cultures by Luminex. An unpaired t-test was performed and significant differences between PBS- and BAP1-treated mice were calculated (* p ≤ 0.05).
Article Snippet: Antigen-specific IgA, IgE, IgG-Fc, IgG1, and IgG2a antibody levels were detected with Bethyl reagents (Bethyl Laboratories, Inc), according to the manufacturer’s recommendations.
Techniques: Enzyme-linked Immunosorbent Assay, Luminex